Isolation and identification of a novel toxin in scallop mantle tissue

Scallops are one of the main marine products Hokkaido, Japan. In addition to adductor muscle, scallop mantle tissue is often consumed in Previously, we showed that feeding mice a diet containing 1% resulted lower food consumption and, ultimately, death. this study, isolated and identified toxic substances from tissue. The were protein complexes with molecular weights 18 kDa 29 kDa. Feeding 0.05% led their death at five weeks. Based on LC-MS/MS analysis, 29-kDa 18-kDa proteins as an actin fragment N-terminal gelsolin-like protein, respectively. was expressed mantle, gill, ovary but not testis, or midgland. Toxicity observed only mouse tissues expressing protein. did induce decreased death, implying both essential for toxicity. This first study identify novel toxin tissues.